American Society of Hematology

Figure 3

$Figure 3. Effects of the different fractions of POP obtained by HPLC separation on the occurrence of cell death. (A) HPLC elution profile of a UVA-irradiated solution of psoralen (32 J/cm2). The eluate was divided in 6 different fractions (A-F) that were evaluated for their apoptogenic activity. (B) Jurkat cells (106/mL) were incubated in the dark with the different HPLC fractions of POP for 20 hours at 37°C. Necrosis and apoptosis were assessed as described in “Materials and Methods” and in Figure 1B. (C) Western blot analysis of PARP from cells incubated under the same conditions described for panel B. PARP cleavage provides further evidence of apoptosis.$

Effects of the different fractions of POP obtained by HPLC separation on the occurrence of cell death. (A) HPLC elution profile of a UVA-irradiated solution of psoralen (32 J/cm²). The eluate was divided in 6 different fractions (A-F) that were evaluated for their apoptogenic activity. (B) Jurkat cells (10⁶/mL) were incubated in the dark with the different HPLC fractions of POP for 20 hours at 37°C. Necrosis and apoptosis were assessed as described in “Materials and Methods” and in Figure 1B. (C) Western blot analysis of PARP from cells incubated under the same conditions described for panel B. PARP cleavage provides further evidence of apoptosis.

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