Functional studies on PBMCs. (A; top) Cytosolic aconitase activity in P and control. (Bottom) IRP1 conformation studies performed as described in Figure 2B. (B; top) H ferritin levels by ELISA. (Bottom) Western blotting of TfR. The vertical white line indicates a deleted lane in the same blot. Equal loading was verified by β-actin. (C) Mitochondrial aconitase activity as measured spectrophotometrically and normalized on mitochondrial malate dehydrogenase (MDH) activity. The experiments were repeated twice. Representative pictures are shown. Error bars represent standard deviations. *P < .01.