Role of PKC-θ in thrombus formation in vivo. Using the FeCl₃ carotid artery injury model of in vivo thrombosis (2 minutes of exposure to 10% FeCl₃), we analyzed the percentage of mice forming stable thrombi (A) and the average time of occlusion (B) in PKC-θ^−/− and WT mice. PKC-θ^−/− mice demonstrated prolonged average occlusion time (mean ± SEM; 15.7 ± 3.3 minutes) and a failure to form a stable thrombus versus littermates (7.6 ± 1.1 minutes of occlusion time; P < .05 by unpaired Student t test). Fisher exact probability test was used for analyzing data shown in panel A.