Sensitivity and specificity of aGvHD pattern. (A) Box-and-whisker plots of classification factor F obtained for classification of each individual HSCT sample (GvHD indicates graft-versus-host disease diagnosed according to Przepiorka et al²⁶ ; control, HSCT sample showing no evidence of GvHD) in the training set and the blinded set (Table 1). The boxes depict the quartiles Q₁ and Q₃ of each distribution, and the statistical medians are shown as horizontal lines in the boxes. The whiskers indicate 3/2 times the interquartile range of Q₁ and Q₃. (B) ROC curve (bold line, AUC = 0.85) of the proteomics pattern diagnosis. Using the GvHD-specific polypeptide panel from Table 3, classification factor F is used as variable in ROC analysis in the 599 samples of the blinded set (Table 1). Ninety-five percent confidence intervals (95% CI) are indicated by thin lines.