Examples of time courses of the proteomics diagnosis based on the aGvHD pattern. The SVM-based classification factor F (F_GvHD) is plotted against the days before and after HSCT starting prior to conditioning treatment (day −10). The threshold was chosen at classification factor F_GvHD = 0.2, as indicated by a line; values above indicate GvHD. The resulting curve is shown for individual patients. (A) Patient 2791 was diagnosed with GvHD grade II on day +27. The classification factor score was 0.8 on day +20 after HSCT, 7 days prior to clinical diagnosis, and returned to inconspicuous values at day +41, reflecting response to treatment. (B) Patient 2725 was diagnosed with GvHD grade III of the skin on day +25 after HSCT. Classification factor F rose to 0.5, thus indicating aGvHD. The initial response to steroid treatment led to the normalization of the factor value on day +55, with no change due to a reactivation of CMV at about day +60 after HSCT. The next rise of the classification factor was concerted with cGvHD (BOOP). (C) Patient 3264 did not develop aGvHD, thus representing an example of a negative control. The values remain around baseline well below the aGvHD cutoff number of 0.2 throughout the observation period.