Binding specificity of LSECtin in dendritic cells and transfectants. Whole cell lysates from K562 cells stably transfected with full-length LSECtin(LSECtin), the Δ3/4 isoform (LSECtin Δ3/4), DC-SIGN, or mock-transfected (Mock) were loaded onto Mannan- (A), N-acetyl-glucosamine- (NAcGlc, B), or N-acetyl-galactosamine (NAcGal)-agarose (C) and retained proteins eluted with SDS sample buffer (SDS-eluted) and analyzed by Western blot using the ADS-1 anti-LSECtin polyclonal antiserum. As a control, aliquots from nonretained material (supernatant) were analyzed in parallel. (D) Whole cell lysates from monocyte-derived dendritic cells were loaded onto Mannan- or N-acetyl-glucosamine- (NAcGlc)-agarose and retained proteins eluted with SDS sample buffer (SDS-eluted) and analyzed by Western blot using polyclonal antisera against LSECtin (ADS-1) or DC-SIGN (DSG1).