Convection-diffusion processes in coagulation. Immunostaining of platelet GPIbα and fibrin(ogen) after 5-minute whole blood perfusion over “center lane” array at 100 s−1. (A) Bottom arrow indicates column where collagen and 25 molecules-TF/μm2 spots were printed (scale bar = 1 mm). Magnified images are of the regions indicated by the white squares (scale bar = 300 μm). Identical results observed in 3 separate experiments. Growth of fibrin width from center of “center lane” plotted against distance downstream from first spot (B). Steady-state numerical simulation of thrombin at 10 μm above the surface for the center lane experiment shown in panel A using constant flux of thrombin from each microarray feature into a flow field containing ATIII (see Table 1 for parameters) (C). Magnified images are the same areas as those in panel A.
