American Society of Hematology

Figure 1

$Figure 1. Non hemolytic antigen loss is not a general property of murine RBCs. (A) C57BL/6 mice were passively immunized with polyclonal anti-HEL antiserum or an anti-HEL IgG fraction from polyclonal antiserum. Control mice received no antibody. Mice were then transfused with a mixture of mHEL RBCs labeled with DiI and C57BL/6 RBCs labeled with DiO. Peripheral blood was obtained at the indicated time points and survival of the RBCs was determined by flow cytometry. (B) C57BL/6 mice were injected with the 10F7 anti-hGPA monoclonal antibody. Control mice received an IgG anti-HEL monoclonal antibody. Mice were then transfused with a mixture of hGPA RBCs labeled with DiI and C57BL/6 RBCs labeled with DiO and RBC survival was monitored. (C-H) Peripheral blood from the mice in panel A was stained with polyclonal anti-HEL antiserum followed by fluorescently labeled anti–mouse IgG and anti-HEL staining was measured by flow cytometry. Error bars in panels A and B represent standard deviation (SD). This experiment was reproduced 3 times with similar results. Representative flow plots and histograms are shown. In panels C, E, and G, the numbers in the upper left and lower right quadrants represent the percentage of circulating transfused B6 or mHEL RBCs, respectively. The numbers in the upper right quadrants represent the ratio of mHEL RBCs to B6 RBCs.$

Non hemolytic antigen loss is not a general property of murine RBCs. (A) C57BL/6 mice were passively immunized with polyclonal anti-HEL antiserum or an anti-HEL IgG fraction from polyclonal antiserum. Control mice received no antibody. Mice were then transfused with a mixture of mHEL RBCs labeled with DiI and C57BL/6 RBCs labeled with DiO. Peripheral blood was obtained at the indicated time points and survival of the RBCs was determined by flow cytometry. (B) C57BL/6 mice were injected with the 10F7 anti-hGPA monoclonal antibody. Control mice received an IgG anti-HEL monoclonal antibody. Mice were then transfused with a mixture of hGPA RBCs labeled with DiI and C57BL/6 RBCs labeled with DiO and RBC survival was monitored. (C-H) Peripheral blood from the mice in panel A was stained with polyclonal anti-HEL antiserum followed by fluorescently labeled anti–mouse IgG and anti-HEL staining was measured by flow cytometry. Error bars in panels A and B represent standard deviation (SD). This experiment was reproduced 3 times with similar results. Representative flow plots and histograms are shown. In panels C, E, and G, the numbers in the upper left and lower right quadrants represent the percentage of circulating transfused B6 or mHEL RBCs, respectively. The numbers in the upper right quadrants represent the ratio of mHEL RBCs to B6 RBCs.

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