The expansion of FluM1-specific CD8⁺ T cells in TIV-vaccinated humanized mice. Experiment as in Figure 1, except that mice received TIV vaccine. (A) Specific CD8⁺ T cells binding FluM1-tetramer and HIVgag-tetramer by flow cytometry. Representative mice from an experiment with 4 mice vaccinated with TIV. (B) The frequency of FluM1-specific CD8⁺ T cells in the blood at day 12 after vaccination from 5 experiments with 4 different donors. Two-tailed nonparametric Mann-Whitney test. (C) CD8⁺ T cells in the spleen and lungs at day 14 after vaccination.