Chemokines induce podosome dissolution in DCs. (A) Untreated or PTX-treated DCs were exposed to the indicated supernatants, or to recombinant chemokines (CCL3, CCL4), and then labeled with antivinculin Ab (green) and phalloidin (red). Bar, 10 μm. (B,C) Cells presenting with podosomes were quantified as in Figure 6. Means plus or minus SEM of 4 independent experiments are shown; at least 25 total cells were examined for each condition in each experiment. Significance was assessed by Student paired t test (*P < .05). (D) Flow cytometric analysis of DC maturation markers in response to chemokines.