Distinct effects of mouse soluble hemojuvelin on BMP2, 4, and 9 induction of hepcidin production. Primary mouse hepatocytes were isolated from wild-type C57BL/6J mice. Hepatocyte cultures were treated with a combination of either 10 μg/mL msHJV (○) or its solvent (●) with 10-fold serial dilutions of (A) BMP2, (B) BMP4, or (C) BMP9 ranging from 0.01 to 100 ng/mL. Means and standard deviations are shown.