Basophil-derived RA modulates IL-3–induced gene expression in an autocrine manner. RA-dependent regulation of CD25, GzmB, and RARα expression in IL-3–stimulated basophils. Basophils were cultured ± IL-3 (20 ng/mL) for 24 hours. Retinal (5 nM), RA (10 nM), or AGN (100 nM) was added as indicated. (Top panel) Surface CD25 expression (CD25) was analyzed by flow cytometry. (Middle panel) GzmB expression was analyzed by ELISA. Bar charts represent the mean values (± SD) of triplicates of representative experiments of 3 shown. (Bottom panel) Trk (NFGR), RALDH2, RARα, and GzmB expression was analyzed by Western blotting. Separate panels represent data from individual experiments with cells from different donors.