Expression of Bcl-2, Mcl-1, A1, and Pin-1 kinase in Bcl-x–deficient B-ALL. (A) Western blot analysis of protein lysates from pleural effusion isolated from B-ALL (lanes 1-3) and Bcl-x–deficient B-ALL mice (lanes 4-6). *Actin response from previous stain. (B) mRNA expression levels of A1 and pim1 in cells isolated from pleural effusion were determined by real-time PCR (results are given as mean ± SEM; n = 4).