Down-regulation of Kpm/Lats2 renders cells resistant to DNA damage–inducing agents. (A) Kpm/Lats2-knockdown cells were established in KG-1a or ED-40515⁺ cells using retrovirus (RV) vector containing Kpm/Lats2-specific shRNA. Wild type represents non–RV-transduced cells and control iRNA represents control shRNA-containing RV–transduced cells. Efficiency of Kpm/Lats2-specific shRNA was measured by real-time PCR analyses. Data normalized to gapdh are shown as mean plus or minus SD in scale that the value for wild type is 1. Analyses were performed in triplicate independently twice and representative data are shown. Western blot analysis of Kpm/Lats2 in wild-type, knockdown, or control cells in KG-1a. Analyses were performed independently twice and representative data are shown. (B) Simple growth curve without agents was measured by MTT assay. The assays were performed in quadruplicate independently 3 times and representative data are shown as mean plus or minus SD. (C) Cell viability after treatment with doxorubicin (DXR) or etoposide (ETP) in each KG-1a line was measured by MTT assay. The assays were performed in quadruplicate independently 3 times and representative data are shown as mean plus or minus SD (Welch t test: *P < .01; **P < .05). (D) Cell viability after treatment with DXR or ETP in each ED-40515⁺ line was measured by MTT assay (Welch t test: *P < .05; **P < .01; ***P < .005). The assays were performed independently in quadruplicate 3 times, and representative data are shown as mean plus or minus SD.