Kpm/Lats2 can associate with YAP2 via its first WW domain and phosphorylate YAP2, but it requires not this association but kinase function of Kpm/Lats2 to stabilize p73. (A) Any one of HA-tagged Kpm-wild type (wt), Kpm-kinase dead (kd), or mock was cotransfected with any one of FLAG-tagged YAP2-wild type (wt), YAP2-S127A (mutant form S127 to A; S127 is Akt-phosphorylation site), YAP2-1WW* (mutant form of first WW domain), YAP2-2WW* (mutant form of second WW domain), or mock into 293T cells by the calcium phosphate method. Upper lanes represent Western blotting with anti-HA in cell lysates. Middle lanes and lower lanes represent Western blotting with anti-FLAG and anti-HA, respectively, in the immunoprecipitates by anti-FLAG. Three independent experiments were performed and representative data are shown. (B) The coimmunoprecipitate fraction by anti-FLAG from each lysate was treated with or without CIP and then analyzed by Western blotting with anti-YAP polyclonal antibody and anti–phospho-YAP (Ser127) polyclonal antibody. Arrow indicates mobility shift band of YAP2. Three independent experiments were performed and representative data are shown.