Effects of ω-3 PUFAs on tube formation. HUVECs were seeded on Matrigel layers, exposed to the indicated treatments (n = 6), and incubated for 20 hours. (A) The formed tubes were detected by phase-contrast inverse microscopy and digital photography. (B) Average lengths of tubes in treatments with PUFAs (10 μM) and indomethacin (IND; 5 μM) were determined by analysis of digital images. *P < .05; ***P < .001 different from control.