Differential effects of ω-6 and ω-3 prostaglandins on proangiogenic processes in HUVECs. (A) Comparative structures of PGE₂ and PGE₃ showing the additional double bond in PGE₃. (B) Representative Western blot of Ang2 analysis (n = 3). HUVECs were treated with VEGF and bFGF (VF) alone or in combination with PGE₂ or PGE₃ (500 nM). (C) Levels of COX-2 were determined by Western blotting (n = 3) in HUVECs that were treated as in panel B. α-tubulin levels are shown to indicate equal loading. “+” signifies Ang2- and COX-2–positive controls.