Stat-6 phosphorylation in FL and FH. (A,B) Tissue lysates of FL (N = 36) and FH (N = 22) were printed on RPMAs and probed with antibodies for Stat-6 (A) and phosphorylated Stat-6 on Tyr641 (B) as described in “Methods.” Scatterpoints represent relative signal intensity averaged over 3 runs and are depicted on a log10 scale. Median group values for FL and FH are designated with horizontal bars on the scatterplots and are listed beneath each plot, with the P value for comparison of the 2 groups. FL samples representing grade 3B are indicated by ○. (C) A total of 6 samples of FL (G1 indicates grade 1; G2, grade 2; G3, grade 3A) were compared with 4 samples of FH on Western blot, using antibodies for detection of total Stat-6 and phospho–Stat-6 Tyr641. Ramos cells untreated (0) and treated with IL-4 for 30 minutes (mn) and 24 hours (hrs) were used as negative and positive controls for Stat-6 activation. (D) A total of 6 samples of FL were compared with 4 samples of FH on Western blot, using antibodies for detection of total Akt, phospho-Akt Ser473, and Bcl-2. HeLa cell control lysates were used as described in Figure 3C.
