Comparison of BALB/c mice engrafted with bone marrow cells transduced with MPLT487A, MPLW515L, and MPLWT. (A) Kaplan-Meier survival plot of recipient BALB/c mice expressing MPLWT (n = 6), MPLW515L (n = 3), and MPLT487A (n = 6). MPLWT mice were killed without signs of disease. (B) Blood count analysis. MPLWT (n = 6), MPLW515L (n = 3), and MPLT487A (n = 6). (C) Splenomegaly in MPLW515L (n = 3) and MPLT487A (n = 5) mice. The spleens of 4 MPLWT mice were weighted for comparison purposes. Error bars denote SD. (D) Flow cytometric analysis of bone marrow cell suspensions (left panel) and spleen cell suspensions (right panel). It shows an increase of granulocytic cells (Mac1⁺Gr1⁺; first row) and of immature erythroid populations (CD71⁺/Ter119⁺; second row) and an amplification of the megakaryocytic lineage (CD41⁺/CD42⁺; third row), when MPLT487A-transduced cells are compared with the others. These analyses are representative of several: MPLWT (n = 3), MPLW515L (n = 1), and MPLT487A (n = 4).