15d-PGJ2 augments DNA content and enhances proplatelet formation. (A) Meg-01 cells were treated with vehicle or 15d-PGJ2 (10 μM) for 24, 48, or 72 hours. Left histogram shows vehicle-treated Meg-01 cells (72 hours). Right histogram shows 15d-PGJ2–treated Meg-01 cells (72 hours). Bar graph demonstrates that by 72 hours Meg-01 cells in the presence of 15d-PGJ2 exhibit higher DNA contents compared with Meg-01 cells in the presence of vehicle. Results are presented as mean plus or minus SD (P < .05). (B) Primary mouse megakaryocytes were treated with vehicle or 15d-PGJ2 (10 μM) for 24 hours. Left histogram shows vehicle-treated cells (24 hours). Right histogram shows 15d-PGJ2–treated cells (24 hours). Bar graph demonstrates that by 24 hours, cells in the presence of 15d-PGJ2 exhibit higher DNA content compared with cells in the presence of vehicle. Results are presented as mean plus or minus SD (P < .05). (C) Left bar graph shows the number of Meg-CFC colonies. Right bar graph shows the size of Meg-CFC colonies. Results are presented as mean plus or minus SD (P < .05). (D) Bar graph showing the percentage of megakaryocytes exhibiting proplatelet extensions. Results are presented as mean plus or minus SD (P < .05). (E) Immunohistochemical GP1bβ and GPV staining of megakaryocyte progenitor-derived colonies. Left picture shows colonies grown from bone marrow of vehicle-treated mice. Middle picture shows colonies grown from bone marrow of 15d-PGJ2–treated mice. Note proplatelet extensions (p). Far right picture shows a magnification of the proplatelet extensions.
