Ndfip1^−/− animals show increased transferrin saturation. (A) Total iron levels, (B) transferrin saturation, and (C) ferritin levels in the sera from Ndfip1^−/− animals and their wild-type Ndfip1^+/+ counterparts were determined as described in “Immunoprecipitation and immunoblotting.” *Significant increase (P < .05) from Ndfip1^+/+ animals. Data are mean plus or minus SD; n = 4.