Figure 2
Design of the MRP8-miR-CD18 transgene expression vector and generation of transgenic founders. (A) A synthetic miRNA hairpin targeting CD18 was cloned in exon 2 of the hMRP8 cassette containing the hMRP8 promoter. The location of the forward (f) and reverse (r) PCR primers for genotyping is indicated. The forward primer is in the intron between exons 1 and 2, and the reverse primer partially overlaps with the 5′miR flanking region. (B) Genotypes of mice were determined by PCR analysis of tail DNA. The expected PCR product of 300 bp for the transgene was observed in 3 animals. (C) FACs analysis of peripheral blood neutrophils (PBNs) and T cells for CD18 (shaded) and isotype control (open) from #85-Tghi and #88-Tgint founders is shown.
