Analysis of CD18 levels in monocytes, macrophages, and T cells. (A) CD18 and CD11b expression by flow cytometry on peripheral blood monocytes (CD115⁺ population) of both transgenic lines: miR-CD18 #85-Tg^hi (solid line), #88-Tg^int (solid line), and wild-type C57Bl/6 (dotted line). Data for isotype IgG (shaded area) are shown. CD18 knockdown in neutrophils was determined using the average geometric mean fluorescence intensity (MFI) plus or minus SEM (graph, bottom) (n = 6-9 for each strain). Dotted line indicates average MFI in CD18-null mice (n = 4), *P < .005. (B) Analysis of CD18 expression in #85-Tg^hi peritoneal (left panel) and bone marrow–derived (BM; right panel) macrophages. Histograms for miR-CD18 #85-Tg^hi (solid line) and wild-type C57Bl/6 (dotted line) mice, and data using isotype IgG (shaded area) are shown. (C) CD18 and CD11a expression in T cells from lines #85-Tg^hi (solid line), #88-Tg^int (solid line), and wild-type C57Bl/6 (dotted line) are shown.