Validation of microarray results by RT-qPCR. (A) Relative mRNA levels corresponding to representative genes from msiRNA-2–treated (▬) and siRNA-2–treated (▭) HUVECs stimulated with (CD154⁺) T cells for 4, 10, and 16 hours were assessed by real-time RT-qPCR using the housekeeping cyclophilin A gene (CYPA) as a control. Results are mean values plus or minus SEM from 2 independent experiments performed in triplicate. In those genes validated at the 3 stimulation times, the comparative expression kinetics between microarrays (■) and RT-qPCR (▲) in terms of mean fold change is shown. (B) Dot-plot of the expression values in log₂ ratio between microarray hybridization (ordinate) and RT-qPCR (abscissa) from all validated genes irrespective of the stimulation time used. Linear regression demonstrates a significant correlation.