TNF-α hypersensitivity is maintained in primary recipients of mock-transduced Fancc^−/− stem cells 6 months after transplantation. (A) Low-density mononuclear cells from Fancc^−/− MD9-EGFP (n = 6) and WT-MD9-EGFP (n = 5) recipients were cultured in clonogenic assays in triplicate to determine their respective sensitivity to TNF-α at 12 months following transplantation. The number of colonies ranged from 80 to 120 per dish. The genotypes are indicated. Data represent the mean and standard error of the mean growth of all recipients in each respective group. *P < .05 and statistical significance between the 2 experimental groups. (B) Representative histologic analysis of BM and spleen of a recipient reconstituted with Fancc^−/− or WT test cells previously transduced with MD9-EGFP.