Occlusive thrombus formation following ferric chloride arterial injury. Ferric chloride was applied to the surface of the carotid artery of pentobarbital-sedated mice. Arterial flow rates were measured for 30 minutes with a Doppler flow probe (flow rates for each mouse are shown in Figure S1). Mice were scored as having stable occlusive thrombi (■), unstable occlusive thrombi (▒), or no thrombi (□). The number of mice forming occlusive thrombi differs between WT and GSK3β^+/− mice (**P = .01, Fisher exact probability test). The results of 15 WT mice and 10 GSK3β mice are shown.