Hierarchical clustering analysis dendrogram of those 30 genes showing the highest differences between freshly isolated and both purified CD69⁺ and CD69⁻ hCMV-stimulated monoclonal CD4⁺ T-LGL (n = 12 paired cell samples). The relative level of expression of each gene is represented by a color code where red represents an expression higher than the mean and green represents an expression below the mean values. Those genes showing different levels of expression in the distinct cell fractions analyzed are known to be involved in inflammatory (1) and immune (2) responses, cell proliferation and/or cell-cycle progression (3), apoptosis (4), protein synthesis (5), G-protein receptor-mediated cell signaling (6), and the DNA repair and maintenance machinery (7). Of note, both CD69⁺ and CD69⁻ hCMV-stimulated monoclonal CD4⁺ T-LGLs showed a high similarity in their levels of expression for most of the genes displayed in the dendrogram, except for NKG7, PRIM2A, CAPNS2, P2RY14, CCL8, KCNJ2, TFEC, IL22, CD28, BAM32, and FCMD.