Analysis of RRV gene expression in lymphoma of animal 19185. RNA isolated from lymphoma of animal 19185 was analyzed by reverse transcriptase (RT)-PCR to confirm expression of vIL-6 and vFLIP and to analyze expression of ORFs 72 and 73. Lanes 1 and 5 indicate 100 base pair ladder; lane 2, RNA from lymphoma amplified with vIL-6-specific primers; lane 3, same as lane 2, minus RT; lanes 4 and 7, GADPH-specific primers as control for RNA; lane 6, no template control; lane 8, ORF-73–specific primers; lane 9, ORF-72–specific primers; and lane 10, ORF-71–specific primers.