Actions of PFTα in cells from a p53-dysfunctional patient and in normal T lymphocytes. Cells from p53-dysfunctional patient 23 were incubated with 10 μM nutlin 3a (A) or 10 μM fludarabine (B). PFTα (25 μM) was added as shown. Whole cell lysates were analyzed by Western blotting. Cell extracts from patient 9 were included as controls to identify the migration positions of p116 and p85 PARP bands. The complete lack of a p53 response by the cells of this patient are documented in Figure S3. (C) Western blot analysis of PARP in normal T lymphocytes incubated with PFTα and/or nutlin. Similar results were obtained in 2 additional experiments.