American Society of Hematology

Figure 1

$Figure 1. Phagosomal pH increases in human DCs, in contrast to human MØs, after latex bead phagocytosis. (A) DC phagosomal pH kinetics after a 15-minute phagocytosis pulse and the indicated time points of chase. (B) MØ phagosomal pH kinetics after a 15-minute phagocytosis pulse and the indicated time points of chase. Each dot represents a different blood donor. Slashes show average pH. Statistics were performed using the nonparametric Wilcoxon test for paired data. *P < .05; **P < .01. (C) Phagosomal membrane expression of gp91phox and V-H+-ATPase assessed by Western blot in human DCs and MØs, after different time points of chase following magnetic bead phagocytosis and phagosome isolation. Purity of the fractions was assessed by evaluating the expression of gp96, Erk-2, and NF-κB.$

Phagosomal pH increases in human DCs, in contrast to human MØs, after latex bead phagocytosis. (A) DC phagosomal pH kinetics after a 15-minute phagocytosis pulse and the indicated time points of chase. (B) MØ phagosomal pH kinetics after a 15-minute phagocytosis pulse and the indicated time points of chase. Each dot represents a different blood donor. Slashes show average pH. Statistics were performed using the nonparametric Wilcoxon test for paired data. *P < .05; **P < .01. (C) Phagosomal membrane expression of gp91phox and V-H⁺-ATPase assessed by Western blot in human DCs and MØs, after different time points of chase following magnetic bead phagocytosis and phagosome isolation. Purity of the fractions was assessed by evaluating the expression of gp96, Erk-2, and NF-κB.

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