ROS production in human DCs and MØs. ROS production was measured over time using luminol-dependent chemiluminiscence, after stimulation with PMA and/or phagocytosis of luminol-coated beads in DCs (left panels) and MØs (right panels) generated in parallel from a series of healthy volunteers. Thick lines show the average of all experiments. (A) Total ROS production after cell stimulation with 0.5 μg/mL PMA in DCs or MØs from the same donors. (B) DC and MØ phagosomal ROS production after a 15-minute pulse with luminol-coated beads. (C) DC and MØ phagosomal ROS production after a 15-minute pulse with luminol-coated beads followed by the addition of 0.5 μg/mL PMA.