Antigen cross-presentation of melanoma soluble antigens is dependent on NOX2 activity. (A) Control HLA-A2–positive DCs (black) and DPI-treated DCs (white) were pulsed with the indicated amounts of short or long MelanA/MART1 peptides. Cross-presentation was evaluated by measuring the production of IFN-γ by the HLA-A2–restricted CTL clone LT12 after DC-CTL coculture. The average and SD of 4 independent experiments are shown. (B) Control HLA-A2–positive DCs (black) and DPI-treated DCs (white) were pulsed with the indicated amounts of short or long gp100 peptides, as described in “Methods.” Cross-presentation was evaluated by measuring the production of IFN-γ by the HLA-A2–restricted CTL clone LT47 after DC-CTL coculture. The average and SD of 4 independent experiments are shown. (C) Control HLA-A2–positive DCs (black) and CGD-DCs (white) were pulsed with the indicated amounts of short or long MelanA/MART1 peptides, as described in “Methods.” Cross-presentation was evaluated by measuring the production of IFN-γ by the HLA-A2–restricted CTL clone LT12 after DC-CTL coculture. The average and SD of 4 independent experiments are shown for control and CGD-DCs. Statistics were performed using the nonparametric Wilcoxon test for nonpaired data. *P < .05; **P < .01.