Figure 1
Farnesyltransferase inhibitor CP 390392 prevents HDJ-2 prenylation. Th1 T cells were incubated for 24 hours in FTI CP 390392 at the indicated concentrations. Cells (2.5 × 106/lane) were lysed and loaded onto a 10% SDS-PAGE gel. Blotting with HDJ-2 antibodies at an FTI concentration of 1.0 μM revealed a second higher-weight band representing unfarnesylated pre–HDJ-2 protein. Blotting for β-actin was performed as a loading control.
