Gene expression profiles of TP53-regulated genes and their clinical relevance. (A) Two-dimensional unsupervised hierarchical cluster analysis of 85 (rows) TP53-regulated genes in CD138-enriched plasma cells of newly diagnosed MM patients on TT2 (n = 351). The right branch consists of MM samples that have a gene expression profile associated with a high TP53 expression level (horizontal green bar), and the left branch contains MM samples that have a gene expression profile associated with low TP53 expression level (horizontal red bar). Kaplan-Meier estimates of (B) EFS and (C) OS in newly diagnosed MM patients on TT2 show superior 5-year actuarial probabilities of EFS (51% vs 39%; P = .001) and OS (69% vs 46%; P = .001) in the right-branch patients whose 85-gene expression profile was associated with a high TP53 expression level.