PKC-θ promotes T cell LFA-1/ICAM-1 binding during antigen recognition. (A) Fluorescence microscopy images of OT-II/PKC-θ^+/+ and OT-II/PKC-θ^−/− CD4⁺ T cell/B cell conjugates.^66,67  OT-II CD4⁺ T cells were added to LPS-activated B cells (blue) pulsed overnight with or without (unstimulated control) 2 μM of OVA_323-339 and incubated at 37°C for 10, 20, 30, and 40 minutes. After fixation, the conjugates were stained for LFA-1 (red). Bar scale represents 5 μm. (B) Graph represents the percentages of conjugates with polarized LFA-1 staining. These results correspond to the data obtained with 2 independent experiments where at least 40 conjugates in each of 5 micrographic fields were counted. Interaction of genotype and stimulation: F(_4,10) = 4324; P = .028; factorial ANOVA (SPSS).