Rapamycin inhibits endogenous c-Myc mRNA translation in MPRO granulocytes. (A) Polysome profiles generated from cell lysates obtained from MPRO cells treated with 48 hours of EtOH (undifferentiated vehicle control), 10⁻⁶ M AGN194204 (RXR), 80 nM rapamycin (rapa), or 10⁻⁶ M AGN194204 and 80 nM rapamycin (RXR + rapa) normalized by loading equal cell number. (B) Total RNA per cell was measured using a NanoDrop ND-1000 UV-Vis Spectrophotometer. (C) c-MYC mRNA abundance per fraction was determined by qRT-PCR in MPRO cells treated as described in panel A. Values are normalized to c-Myc mRNA abundance in fraction 1 for EtOH-treated cells. Data shown are the mean plus or minus SEM of 3 independent experiments. (D) Distribution of c-Myc mRNA in fractionated lysates was determined by qRT-PCR. Average transcript abundance in each fraction was divided by average net transcript abundance in all fractions and expressed as a percentage of total for the 3 experiments included in panel C.