Figure 2
Culture of human cord blood CD34+ cells in the presence of Angptl5 stimulates ex vivo expansion of SRCs. (A) Culture of 104 fresh human cord blood CD34+ cells was initiated in serum-free STF medium or STF medium supplemented with 500 ng/mL human Angptl5. Total cell numbers were counted. (B) Extent of human chimerism in the bone marrow of NOD/SCID mice that received a transplant of 10 000 fresh human cord blood CD34+ cells, or the progenies of initial 5000 cells cultured for 10 days in STF medium or STF medium supplemented with Angptl5. Each symbol represents the engraftment of a single mouse that underwent transplantation assayed at 2 months after transplantation. *Significantly different from lanes 1 and 2. Student t test, P < .05.
