Lymphoid progenitors from TLR-deficient mice are not primed to become DCs during HSV-1 infection. (A) Total numbers of pre-B and B cells were enumerated in BM of TLR^+/+ and TLR^−/− mice on day 7 of acute infection. Data are means plus or minus SEM (*significant difference, P < .05). CLPs were highly purified from HSV-1–infected and control mice and placed in lymphoid cultures for 8 days. (B) The indicated flow cytometry gates were used to discriminate B220⁺CD19⁻CD11c⁺CD11b⁻ pDCs + NK-like IKDCs and B220⁻CD19⁻CD11c⁺CD11b⁺ cDCs. Data are representative of 2 independent experiments with 3 replicates each.