E2 did not modify the signaling induced by IFNβ. (A) Expression of genes involved in type I IFN signaling and IFN-inducible genes measured by qRT-PCR in DCs pretreated for 24 hours with E2 and treated for 15 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours with 5000 U/mL IFNβ. Data are presented as mean plus or minus SD of 4 independent experiments. (B) IP-10 expression measure by ELISA in DCs pretreated for 24 hours with E2 and treated for 15 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours with 5000 U/mL IFNβ. Data are presented as mean plus or minus SD from 4 independent experiments.