Figure 1
Figure 1. IL-6 and IL-10 are targets of the NF-κB pathway. (A,B) Gene expression patterns of lymphoma cell line OCI-Ly3 and OCI-Ly10 induced to express IkBsr with doxycycline for the indicated times. Each column is a single experiment comparing 2 cDNA populations; the treated sample was labeled red (Cy5) and the untreated sample was labeled green (Cy3). Each row represents data from a single cDNA microarray spot. The red-to-green (Cy5/Cy3) ratio reflects hybridization to that spot, a measure of relative gene expression; intensity reflects the magnitude of the difference between the samples according to the ratio color scale. Red indicates Cy5/Cy3 ratios greater than 1, green indicates Cy5/Cy3 ratios less than 1, black indicates no significant change in gene expression, and gray indicates that the spot did not meet data selection criteria. These ratios were depicted according to the color scale shown at the bottom. The criteria for selecting these genes are described in “Methods.” (C) Expression of IκBsr inhibits IL-6 secretion. OCI-Ly3 cells were induced to express IκBsr with doxycycline. Cells were washed before adding doxycycline. ELISA was used to determine the amount of IL-6 secreted in the medium. (D) Expression of IκBsr inhibits IL-10 secretion. OCI-Ly10 cells were induced to express IκBsr with doxycycline. Cells were washed before adding doxycycline. ELISA was used to determine the amount of IL-10 secreted in the medium. (E) Expression of IL-6 and IL-10 in ABC and GCB DLBCL cell lines was measured using the Quantikine colorimetric sandwich ELISA kit. (F) Steady-state levels of total and phosphorylated STAT3 in ABC and GCB DLBCL cell lines. Cell lysate were separated on Western blotting and immunoblotted with antibodies specific for p-STAT3 (Tyr705), pSTAT3 (Ser727), STAT3, or AKT (control). A vertical line has been inserted to indicate a repositioned gel lane. (G) Relative STAT3 mRNA levels in ABC DLBCL and GCB DLBCL cell lines as assessed by gene-expression profiling on Affymetrix U133 plus 2.0 arrays (probe ID_1123163).

IL-6 and IL-10 are targets of the NF-κB pathway. (A,B) Gene expression patterns of lymphoma cell line OCI-Ly3 and OCI-Ly10 induced to express IkBsr with doxycycline for the indicated times. Each column is a single experiment comparing 2 cDNA populations; the treated sample was labeled red (Cy5) and the untreated sample was labeled green (Cy3). Each row represents data from a single cDNA microarray spot. The red-to-green (Cy5/Cy3) ratio reflects hybridization to that spot, a measure of relative gene expression; intensity reflects the magnitude of the difference between the samples according to the ratio color scale. Red indicates Cy5/Cy3 ratios greater than 1, green indicates Cy5/Cy3 ratios less than 1, black indicates no significant change in gene expression, and gray indicates that the spot did not meet data selection criteria. These ratios were depicted according to the color scale shown at the bottom. The criteria for selecting these genes are described in “Methods.” (C) Expression of IκBsr inhibits IL-6 secretion. OCI-Ly3 cells were induced to express IκBsr with doxycycline. Cells were washed before adding doxycycline. ELISA was used to determine the amount of IL-6 secreted in the medium. (D) Expression of IκBsr inhibits IL-10 secretion. OCI-Ly10 cells were induced to express IκBsr with doxycycline. Cells were washed before adding doxycycline. ELISA was used to determine the amount of IL-10 secreted in the medium. (E) Expression of IL-6 and IL-10 in ABC and GCB DLBCL cell lines was measured using the Quantikine colorimetric sandwich ELISA kit. (F) Steady-state levels of total and phosphorylated STAT3 in ABC and GCB DLBCL cell lines. Cell lysate were separated on Western blotting and immunoblotted with antibodies specific for p-STAT3 (Tyr705), pSTAT3 (Ser727), STAT3, or AKT (control). A vertical line has been inserted to indicate a repositioned gel lane. (G) Relative STAT3 mRNA levels in ABC DLBCL and GCB DLBCL cell lines as assessed by gene-expression profiling on Affymetrix U133 plus 2.0 arrays (probe ID_1123163).

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