Elimination of CTLs occurs early during interaction with κaAPCs. CMVpp65-specific CTLs were cocultured with different loaded or unloaded bead preparations (x-axis). At indicated time points (30, 60, and 120 minutes following initiation of cocultures), beads were magnetically removed and CMVpp65-specific CTLs were further incubated. After 48 hours, numbers of viable CTLs, (% viable cells = (% treated cells × 100%)/% untreated cells were determined as described in the experimental protocols. CMVpp65-specific CTLs cocultured for 48 hours with the indicated beads served as positive control.