PPAR-γ is expressed in human PMNs. Messenger RNA expression of PPAR-γ by isolated PMNs was analyzed by (A) semiquantitative and (B) quantitative RT-PCR in PMNs after treatment (0-8 hours) with TNF-α (20 ng/mL), IL-4 (20 ng/mL), or LPS (100 ng/mL). *P < .05 compared with DMSO (vehicle) treated cells. Results are expressed as the average of 3 independent experiments performed in quadruplicate. Error bars represent SEM. (C) Western immunoblotting after treatment for 16 hours with TNF-α (10 and 20 ng/mL), IL-4 (5 and 10 ng/mL), or LPS (100 ng/mL). Equal loading was confirmed by stripping and probing with antibody against GAPDH. Gel and blot shown are representative of 3 separate experiments.