Figure 2
PPAR-γ ligands decrease human PMN chemotaxis. Cells were isolated and treated with troglitazone (Tro; 1-10 μM) or 15d-PGJ2 (PGJ2; 1-10 μM) for 2 hours before stimulation with (A) 10−7 M fMLP or (B) 100 ng/mL IL-8 for assessment of chemotaxis. All data are expressed as mean PMN count per high-powered field at 100× magnification (PMNs/hpf). *P < .05 compared with DMSO (vehicle) treated cells. Results are expressed as the average of 2 independent experiments performed in triplicate. Ctrl, unstimulated cells (without fMLP or IL-8 treatment). Error bars represent SEM.
