Constitutively active PPAR-γ decreases fMLP-induced HL-60 chemotaxis and ERK-1/2 phosphorylation. (A) DMSO-treated HL-60 cells transiently transfected with either the pcDNA3-VP16-PPAR-γ construct or pcDNA3 (control vector), were stimulated with 10−7 M fMLP and assessed for chemotaxis. All data are expressed as mean HL-60 count per high-powered field at 100× magnification (cells/hpf). *P < .05 compared with cells transfected with control vector (Ctrl). Results are expressed as the average of 3 independent experiments performed in triplicate. Unstim, unstimulated cells without fMLP treatment. (B) Cells transfected as in panel A were lysed 0, 2, 5, and 10 minutes after treatment with 10−7 fLMP, and phosphorylation of ERK-1/2 was determined by Bio-Plex assay. The ratio of phosphorylated to total ERK-1/2 is displayed. No phosphorylation of ERK-1/2 was observed at 10 minutes. *P < .05 compared with cells transfected with control vector (Ctrl). Results are expressed as the average of 3 independent experiments performed in triplicate. Error bars represent SEM.
