Plasmin enhances HPC mobilization after G-CSF. (A,B) Compared with WT mice, AP^−/− mice had more circulating CFU-C (A) and CFU-S (B) at 5 days after G-CSF. *P < .05 vs WT (n = 10-15). (C,D) Compared with vehicle, treatment with 100 mg/kg tenecteplase (TNK, daily intraperitoneal) or 100 μg/day microplasmin (μPli, osmotic minipump) during 5 days increased the number of circulating CFU-C (C) and CFU-S (D), mobilized in response to G-CSF. Vehicle groups behaved similarly (not shown) and were therefore pooled together. *P < .05 vs WT (n = 10-15). (E) More lethally irradiated syngeneic WT recipients survived when they were transplanted with 1 × 10⁵ PB-MNCs from G-CSF–treated WT mice, receiving TNK or μPli than vehicle (P < .05, Cox regression; n = 13-20).