Detection of gene products. (A) Western blots of RBC ghost proteins demonstrating loss of both β- and γ-adducin in α-adducin knockout mice. (B) Northern blots of E14.5 fetal livers showing loss of α-adducin mRNA but normal levels of β- and γ-adducin mRNA. The exon 10 to 12 α-adducin probe was used (Table 1). (C) Western blots demonstrating loss of α-adducin in all tissues examined. (D) Western blots demonstrating loss of platelet γ-adducin in α-adducin–null mice. +/+ indicates wild-type; +/−, heterozygotes; and −/−, homozygous null.