miR-26a–induced phenotypic changes in a MYC expression background. (A) At indicated time points, cell count analysis was performed after induction of miR-26a expression in transfected Raji and Namalwa cells. After 72 hours of miR-26a overexpression, cell numbers were reduced in Raji and Namalwa cells (P = .031, exact Wilcoxon rank sum test). These effects were more pronounced after 96 hours of miRNA induction (P = .031, exact Wilcoxon rank sum test). As a control, empty vector- containing cells were treated with Dox for the same time periods. (B) Cell-cycle analysis in empty-vector and miR-26a-transfected Raji and Namalwa cells was determined by PI staining at indicated time points after Dox treatment. Compared with the empty-vector control, there was an increased percentage of cells in G1 phase within the miR-26a–expressing cell lines (P = .031, exact Wilcoxon rank sum test).
