Role of SCF in migration and signaling activation in F/P-expressing BMMCs. (A) Time course of SCF-induced activation of Akt (p-Akt), Erk (p-Erk), STAT5 (p-STAT5), c-kit (p-c-Kit), and overall tyrosine phosphorylation (p-Tyr) in mock vector–transduced and F/P-expressing MCs. β-Actin is used as loading control. The numbers represent the densitometric quantification of p-AKT relative to β-actin in this experiment. One representative experiment (of 3-4 completely independent experiments) is shown. (B) Migration of F/P-expressing BMMCs toward stem cell factor (SCF). F/P-expressing (■) or mock vector–transduced (□) BMMCs were allowed to migrate in response to a gradient of different concentrations of SCF. Data represent means (± SEM) of BMMCs that migrated into lower chambers. *P < .05, compared with mock vector–transduced in each concentration of SCF; #P < .05, compared with medium control in each group (n = 3, from 1 representative experiment of 3 independent experiments with similar results).