Increasing lymphoid transcriptional priming in LMPPs expressing Rag1^GFP. (A) Coexpression pattern of Rag1^GFP and Flt3 on Kit-enriched LSK BM cells. Gates denote the sorting strategies used to purify LSKFlt3^hiRag1^GFP− (Rag1^GFP−), LSKFlt3^hiRag1^GFPlo (Rag1^GFPlo), LSKFlt3^hiRag1^GFPint (Rag1^GFPint), LSKFlt3^hiRag1^GFPhi (Rag1^GFPhi), and LSKFlt3⁻ (Flt3⁻) cells. Percentages indicate mean frequencies of the sorted populations of total LSK cells from 4 experiments. Right panels show typical purity analysis. (B) Expression pattern of Rag1 transcripts in single cells from sorted BM LSKFlt3⁻, LSKFlt3^hiRag1^GFP−, LSKFlt3^hiRag1^GFPlo, LSKFlt3^hiRag1^GFPint and LSKFlt3^hiRag1^GFPhi cells. Mean plus or minus SEM values from 2 experiments with 88 single cells of each cell population investigated in each experiment, except for LSKFlt3⁻ cells, in which 88 cells were evaluated in total. (C) Expression pattern of individual lymphoid genes (Il7r, sterile IgH transcript [IgH], and Rag1) in single cells from BM LSKFlt3^hiRag1^GFP−, LSKFlt3^hiRag1^GFPlo, LSKFlt3^hiRag1^GFPint, and LSKFlt3^hiRag1^GFPhi cells. To the right is shown the frequency of cells in each population coexpressing 2 or 3 of the investigated lymphoid genes (Multi-Ly). Mean plus or minus SEM values from 2 experiments with 88 single cells of each cell population investigated in each experiment, except for LSKFlt3⁻ cells, in which 88 cells were evaluated in total.