Figure 6
Figure 6. LSKFlt3hi cells with increasing levels of transcriptional lymphoid priming continue to coexpress GM genes. Coexpression patterns of transcriptional lineage programs in single cells from BM LSK subpopulations separated based on levels of Rag1GFP expression. Cells were scored as expressing GM and/or lymphoid (Ly) programs based on the expression of one or more lineage-associated genes: GM: Csf3r and Mpo; lymphoid: Rag1, sterile IgH transcript, and Il7r. Mean plus or minus SEM values from 2 experiments with 88 single cells of each cell population investigated in each experiment, except for LSKFlt3− cells, in which 88 cells were evaluated in total.

LSKFlt3hi cells with increasing levels of transcriptional lymphoid priming continue to coexpress GM genes. Coexpression patterns of transcriptional lineage programs in single cells from BM LSK subpopulations separated based on levels of Rag1GFP expression. Cells were scored as expressing GM and/or lymphoid (Ly) programs based on the expression of one or more lineage-associated genes: GM: Csf3r and Mpo; lymphoid: Rag1, sterile IgH transcript, and Il7r. Mean plus or minus SEM values from 2 experiments with 88 single cells of each cell population investigated in each experiment, except for LSKFlt3 cells, in which 88 cells were evaluated in total.

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